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sf 21 cells  (Expression Systems Inc)


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    Expression Systems Inc sf 21 cells
    Sf 21 Cells, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 99/100, based on 793 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sf21+cells/Sf21+Cells/us12630528-831-14-25
    Average 99 stars, based on 793 article reviews
    sf 21 cells - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Deciphering molecular determinants of GPBAR1-Gs protein interactions by HDX-MS and cryo-EM
    Article Snippet: .. Sf21 cells were cultured in SFM Sf-900 II medium (Gibco) at 28 °C to a density of 4.5 × 106 cells/ml and then were co-infected with GPBAR1, Gαs and Gβ1γ2 using Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific) at the ratio of 1:1:1. ..

    Article Title: Structure and function of IWS1 in transcription elongation
    Article Snippet: .. Sf9 cells (Expression Systems, Cat# 94-001S), T . ni (Hi5) cells (Expression Systems, Cat# 94-002S), and Sf21 cells (Expression Systems, Cat# 94-003S) were cultured in ESF 921 insect cell culture medium (Expression Systems, Cat# 96-001-01) at 27°C for baculovirus production and protein expression, unless otherwise indicated. .. E. coli DH5α (New England Biolabs (NEB) Cat# C2987H), DH10EMBacY (Geneva Biotech), and BL21-CodonPlus (DE3)-RIL (Agilent Cat# 230245) were cultured in LB broth (EMD-Millipore Cat# 71753-5) at 37°C for plasmid production and protein expression, unless otherwise indicated.

    Expressing:

    Article Title: Structure and function of IWS1 in transcription elongation
    Article Snippet: .. Sf9 cells (Expression Systems, Cat# 94-001S), T . ni (Hi5) cells (Expression Systems, Cat# 94-002S), and Sf21 cells (Expression Systems, Cat# 94-003S) were cultured in ESF 921 insect cell culture medium (Expression Systems, Cat# 96-001-01) at 27°C for baculovirus production and protein expression, unless otherwise indicated. .. E. coli DH5α (New England Biolabs (NEB) Cat# C2987H), DH10EMBacY (Geneva Biotech), and BL21-CodonPlus (DE3)-RIL (Agilent Cat# 230245) were cultured in LB broth (EMD-Millipore Cat# 71753-5) at 37°C for plasmid production and protein expression, unless otherwise indicated.

    Article Title: Mechanism of co-transcriptional cap snatching by influenza polymerase.
    Article Snippet: .. Initial viruses were generated using transfection in Sf9 cells (obtained from ThermoFisher, not verified in-house), virus propagation in Sf21 cells and protein expression in Hi5 cells (both obtained from Expression Systems, not verified in-house). .. Instead of ammonium sulfate precipitation as a first step during purification, the supernatant was clarified by ultracentrifugation in a Ti45 rotor (Beckman Coulter) at 45,000 rpm and 4 °C for 1 h. To generate the human transcription factor DSIF with altered binding interfaces with FluPol, SPT5 mutations were introduced.

    Article Title: Mechanism of co-transcriptional cap snatching by influenza polymerase
    Article Snippet: .. Initial viruses were generated using transfection in Sf9 cells (obtained from ThermoFisher, not verified in-house), virus propagation in Sf21 cells and protein expression in Hi5 cells (both obtained from Expression Systems, not verified in-house). .. Instead of ammonium sulfate precipitation as a first step during purification, the supernatant was clarified by ultracentrifugation in a Ti45 rotor (Beckman Coulter) at 45,000 rpm and 4 °C for 1 h. To generate the human transcription factor DSIF with altered binding interfaces with FluPol, SPT5 mutations were introduced.

    Amplification:

    Article Title: RNA virus polymerase-helicase coupling enables rapid elongation through duplex RNA.
    Article Snippet: The bacmid was transfected into Sf9 cells (Expression Systems) with Cellfectin II Reagent (Life Technologies) to generate recombinant baculoviruses. .. The baculoviruses were amplified through two passages in Sf9 cells, and then used to infect 1 L of Sf21 cells (Expression Systems), in which they were incubated for 48 h at 27◦C. .. The cells were harvested by centrifugation, resuspended in wash buffer (25 mM HEPES pH 7.4, 300 mM NaCl, 1 mM MgCl2, 5 mM DTT) with 143 μL of BioLock (IBA LifeSciences) per liter of culture.

    Incubation:

    Article Title: RNA virus polymerase-helicase coupling enables rapid elongation through duplex RNA.
    Article Snippet: The bacmid was transfected into Sf9 cells (Expression Systems) with Cellfectin II Reagent (Life Technologies) to generate recombinant baculoviruses. .. The baculoviruses were amplified through two passages in Sf9 cells, and then used to infect 1 L of Sf21 cells (Expression Systems), in which they were incubated for 48 h at 27◦C. .. The cells were harvested by centrifugation, resuspended in wash buffer (25 mM HEPES pH 7.4, 300 mM NaCl, 1 mM MgCl2, 5 mM DTT) with 143 μL of BioLock (IBA LifeSciences) per liter of culture.

    Construct:

    Article Title: An Intrinsically Disordered Region of Histone Demethylase KDM5A Activates Catalysis Through Interactions With the Nucleosomal Acidic Patch and DNA
    Article Snippet: Spodoptera frugiperda (Sf21) cells (Expression Systems 94–003S) were cultured in ESF921 media with no additives (Expression Systems 96–001) in suspension at 27 °C, rotating at 125 rpm. .. All KDM5A catalytic constructs were expressed in Sf21 cells using a protocol adapted from the Gibco Bac-to-Bac Baculovirus Expression system and previously described [ ]. .. KDM5A sequences were cloned using NEB HiFi DNA Assembly (NEB E2621) into an empty pFastBac vector (pFB-LIC-Bse, Opher Gileadi, Addgene plasmid # 26108) containing a 6xHis tag and TEV cleavage site.

    Generated:

    Article Title: Mechanism of co-transcriptional cap snatching by influenza polymerase.
    Article Snippet: .. Initial viruses were generated using transfection in Sf9 cells (obtained from ThermoFisher, not verified in-house), virus propagation in Sf21 cells and protein expression in Hi5 cells (both obtained from Expression Systems, not verified in-house). .. Instead of ammonium sulfate precipitation as a first step during purification, the supernatant was clarified by ultracentrifugation in a Ti45 rotor (Beckman Coulter) at 45,000 rpm and 4 °C for 1 h. To generate the human transcription factor DSIF with altered binding interfaces with FluPol, SPT5 mutations were introduced.

    Article Title: Mechanism of co-transcriptional cap snatching by influenza polymerase
    Article Snippet: .. Initial viruses were generated using transfection in Sf9 cells (obtained from ThermoFisher, not verified in-house), virus propagation in Sf21 cells and protein expression in Hi5 cells (both obtained from Expression Systems, not verified in-house). .. Instead of ammonium sulfate precipitation as a first step during purification, the supernatant was clarified by ultracentrifugation in a Ti45 rotor (Beckman Coulter) at 45,000 rpm and 4 °C for 1 h. To generate the human transcription factor DSIF with altered binding interfaces with FluPol, SPT5 mutations were introduced.

    Transfection:

    Article Title: Mechanism of co-transcriptional cap snatching by influenza polymerase.
    Article Snippet: .. Initial viruses were generated using transfection in Sf9 cells (obtained from ThermoFisher, not verified in-house), virus propagation in Sf21 cells and protein expression in Hi5 cells (both obtained from Expression Systems, not verified in-house). .. Instead of ammonium sulfate precipitation as a first step during purification, the supernatant was clarified by ultracentrifugation in a Ti45 rotor (Beckman Coulter) at 45,000 rpm and 4 °C for 1 h. To generate the human transcription factor DSIF with altered binding interfaces with FluPol, SPT5 mutations were introduced.

    Article Title: Mechanism of co-transcriptional cap snatching by influenza polymerase
    Article Snippet: .. Initial viruses were generated using transfection in Sf9 cells (obtained from ThermoFisher, not verified in-house), virus propagation in Sf21 cells and protein expression in Hi5 cells (both obtained from Expression Systems, not verified in-house). .. Instead of ammonium sulfate precipitation as a first step during purification, the supernatant was clarified by ultracentrifugation in a Ti45 rotor (Beckman Coulter) at 45,000 rpm and 4 °C for 1 h. To generate the human transcription factor DSIF with altered binding interfaces with FluPol, SPT5 mutations were introduced.

    Virus:

    Article Title: Mechanism of co-transcriptional cap snatching by influenza polymerase.
    Article Snippet: .. Initial viruses were generated using transfection in Sf9 cells (obtained from ThermoFisher, not verified in-house), virus propagation in Sf21 cells and protein expression in Hi5 cells (both obtained from Expression Systems, not verified in-house). .. Instead of ammonium sulfate precipitation as a first step during purification, the supernatant was clarified by ultracentrifugation in a Ti45 rotor (Beckman Coulter) at 45,000 rpm and 4 °C for 1 h. To generate the human transcription factor DSIF with altered binding interfaces with FluPol, SPT5 mutations were introduced.

    Article Title: Mechanism of co-transcriptional cap snatching by influenza polymerase
    Article Snippet: .. Initial viruses were generated using transfection in Sf9 cells (obtained from ThermoFisher, not verified in-house), virus propagation in Sf21 cells and protein expression in Hi5 cells (both obtained from Expression Systems, not verified in-house). .. Instead of ammonium sulfate precipitation as a first step during purification, the supernatant was clarified by ultracentrifugation in a Ti45 rotor (Beckman Coulter) at 45,000 rpm and 4 °C for 1 h. To generate the human transcription factor DSIF with altered binding interfaces with FluPol, SPT5 mutations were introduced.



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